94 Changes in Vaginal Microbiome of Beef Cows Enrolled in an Estrous Synchronization Protocol
نویسندگان
چکیده
Abstract Vaginal pH and microbiome have been reported to influence fertility in women. In cattle, estrous synchronization protocols are a great tool overcome infertility; however, the effects of hormones administered protocol on vaginal unknown. A total 24 cows were enrolled 7-d Co-Synch + controlled internal drug release (CIDR) had individual flushes collected during handling events days 0, 7 day 10 at timed-artificial insemination (TAI). Flushes used for analysis DNA extraction. Extracted was submitted by whole genome shotgun sequencing. Pregnancy status assessed ultrasonography 35 after TAI. Firmicutes most abundant phylum, followed Proteobacteria, Actinobacteria all collection days. Bacteria from Clostridia genus samples. Greater alpha Shannon diversity (within sample diversity) observed pregnant animals -10 compared with non-pregnant class, order (P < 0.01), as well family, level 0.001). Overall, decreased 0 0.05 class level; P< 0.01 family level) greater TAI P 0.001 level). No differences between samples D0 > 0.05). correlations relation 0.1). Bray-Curtis (between revealed clustering order, tendency (0.1 0.05) phylum level. conclusion, within prior initiation. Furthermore, bacterial changes throughout protocol, but restored day0
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 2023
ISSN: ['0021-8812', '1525-3163', '1525-3015', '1544-7847']
DOI: https://doi.org/10.1093/jas/skad068.072